Morphologic study of pollens of three cultivated Actinidia species by scanning electron microscopy
QI Xiujuan
WANG Ran
LAN Yanping
CHEN Jinyong
GU Hong
FANG Jinbao
Abstract：[Objective]In order to obtain the knowledge of genetic variation and relationship of various cultivars in the genus Actinidia Lindl.,the pollen morphological characteristics of three Actinidia species were investigated.[Methods] Scanning electron microscopy was used to observe pollen morphology of thirty samples collected from three cultivated Actinidia species,A.deliciosa,A.chinensis and A.arguta.Parameters of pollen grains such as the length of polar axis,length of equatorial axis,length of colpus and width of colpus were measured and analyzed using an image software (Imagepro-Plus).[Results] The pollens of A.deliciosa,A.chinensis and A.arguta were in the form of a single grain,being prolate or perprolate.Only one sample ofA.deliciosa contained the prolate-shaped pollen grains whereas all samples of A.chinensis contained the perprolate-shaped pollen grains.A total of ten samples of A.arguta were included in this study,four of them contained the prolate-shaped pollen grains while others contained perprolateshaped pollen grains.Each pollen grain contained three colpi which elongated along the polar axis till they reached the ends.One or two colpi could be observed in the equatorial view,whereas all three corpi that were furrows in trifid circular could be seen in the polar view.The results of the measurements of A.deliciosa pollen grain were as follows:The length of colpus was 18.08-31.48 μm and the coefficient variation was 9.59％.The width of colpus was 4.65-11.42 μm and the coefficient variation was 12.40％.The length of polar axis was 21.54-36.05 μm and the coefficient variation was 7.6％.The length of equatorial axis was 9.60-17.66 μm and the coefficient variation was 10.01％.The ratio between the polar and the equatorial axis was 1.65-3.05 and the coefficient variation was 9.68％.The pollen size was 244.90-608.52 μm2 and the coefficient variation was 15.06％.The results of the measurements of A.chinensis pollen grain were as follows:The length of colpus was 14.09-24.96 μm and the coefficient variation was 14.00％.The width of colpus was 4.09-9.37 μm and the coefficient variation was 15.88％.The length of polar axis was 15.60-30.11 μm and the coefficient variation was 11.17％.The length of equatorial axis was 8.85-17.87 μm and the coefficient variation was 11.80％.The ratio between the polar and the equatorial axis was 1.26-2.65 and the coefficient variation was 9.93％.The pollen size was 182.58-443.18 μm2 and the coefficient variation was 21.52％.The results of the measurements of A.arguta pollen grain were as follows:the length of colpus was 16.34-30.06 μm and the coefficient variation was 11.62％.The width of colpus was 6.11-11.23 μm and the coefficient variation was 14.13％.The length of polar axis was 18.66-32.11 μm and the coefficient variation was 9.21％.The length of equatorial axis was 8.76-18.42 μm and the coefficient variation was 10.79％.The ratio between the polar and the equatorial axis was 1.33-2.68 and the coefficient variation was 10.90％.The pollen size was 166.62-547.07 μm2 and the coefficient variation was 16.70％.There are three types of surface ornamentation in Actinidia pollens.(1) The corrugated ornamentation was covered with a smooth surface composed of irregular strips.Sixteen samples showed corrugated ornamentation,which accounted for 53.33％ of the total samples used in this study.Among them,five samples belonged to A.deliciosa,which accounted for 38.46％ of A.deliciosa pollens.Five samples belonged to A.chinensis,which accounted for 71.43％ of A.chinensis pollens.Seven samples belonged to A.arguta,which accounted for 70％ of A.arguta pollens.(2) The pollen with warty ornamentation was covered by regular or irregular block like protrusions.The size of these protrusions was irregular.Eleven samples showed warty ornamentation,which accounted for 36.67％ of all samples included in this study.Eight samples belonged to A.deliciosa,which accounted for 61.54％ of A.deliciosa pollens.Two samples belonged to A.chinensis,which accounted for 10％ of A.chinensis pollens.One sample belonged to A.arguta,which accounted for 10％ ofA.arguta pollens.(3) The surface of pollens with granular ornamentation was distributed with particles or in the pattern of granules.Three samples showed granular ornamentation,which accounted for 10％ of all samples included in this study.The samples with granular ornamentation belonged to A.arguta which was originated from the province of Heilongjiang.It accounted for 30％ of A.arguta pollens.[Conclusion] There was a certain degree of conservation in pollen morphology and size of Actinidia pollens derived from the same species or environmental background.Overall,the pollen size of A.deliciosa was the largest among these species,followed by A.arguta and A.chinensis.The length the polar axis,length of equatorial axis,length of colpus and width of colpus,of A.chinensis were lower than those of A.deliciosa.Hence,the morphological differences of pollens between A.chinensis and A.deliciosa would provide some evidence for taxonomy of these species.
Keywords：KiwifruitPollen morphologyPollen ornamentationGenetic diversity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 1365-1373 )