The signal pathway of melon calli ROS metabolism and defensive enzyme activities induced by BTH
Abstract：The melon calli were cultured in dark （25±2） ℃ on MS medium contained 5 mmol＇L-1 coffeic acid （CA） and/or 10 mmol. L-1 CoCI： （CC）, while benzothiadiazole （BTH） were sprayed on the calli. The production rate of O2-, content of H202 and the activities of SOD, CAT, POD, PPO, PAL were determined. The result showed that CA inhibited the induction of BTH on O2- rate, H2O2 production and CAT decrease. While CC stimulated O2- rate, but had no effect on BTH induced H2O2 production and CAT decrease. Ei- ther CA or CC could partially inhibit the increase of SOD activity induced by BTH, and CA＋CC completely counterbalanced the effect of BTH on SOD. CC eliminated POD increase induced by BTH, while CA showed no effect on this. Both CA and CC promoted the increase of PAL induced by BTH. These results demonstrate that the induction of BTH on CAT activity, O2- rate and H2O2 production is through SA-dependent pathway, the induction of BTH on POD activity is through ET-dependent pathway, and the effect of BTH on SOD activity is through SA- and ET-dependent pathways, while the induction of BTH on PAL may be via signal transduction other than SA- or ET-dependent pathway.
Keywords：MelonBenzothiadiazoleCoffeic acidCobalt chlorideReactive oxygen speciesDefensive enzymesSignal pathway
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)