Cloning and expression analysis of two fragments of HSP70 cDNA in the peel of banana during storage at high and low temperature
Abstract：Many evidences have indicated that temperature stress can induce heat shock protein expression and heat treatment can alleviate chilling injury of fruits. In the present study, two HSP70 fragments were cloned from banana peel using homology-based method in order to investigate their expressions regulated by temperature stress and the relationship between their expressions and chilling injury. Using PCR de- generate primers designed with reference to the conserved amino acid sequences of known HSP70, total RNA extracted from banana peel was used as templates and HSPTO eDNA fragments were amplified by RT-PCR in order to study their expression characteristics under different environments by Northern hy- bridization. Two different eDNA fragments, named Ma-HSP70-1 and Ma-HSP70-2, were cloned. The homology between two HSP70 is 86.9% at nueleotide acid sequence level and 97.1% at amino acid se- quence level. Using Ma-HSP70-1 and Ma-HSP70-2 as probes, Northern hybridization showed that short-time heat pretreatment （38 ℃ ）only induced the expression of Ma-HSP70-2 transcripts, However, storage at low temperature enhanced accumulations of 2 HSP70 mRNA. Ma-HSPTO might be related to chilling tolerance of banana fruits.
Keywords：BananaHSP70eDNA cloningSequenceNorthern hybridization
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)