Analysis of entophytic bacteria community structure in banana by PCR- DGGE technique
Abstract：The total genomic DNA was extracted from various tissues of both the healthy bananas and the ones which were infected with Fusarium oxysporum f. sp. cubense （FOC）. Then the 16Sr DNA V3 frag- ment polymerase chain reaction products amplified from the total genomic DNA were analyzed by denatur- ing gradient gel eleetrophoresis （DGGE）. The results of sequence analysis of the 18 DGGE dominant bands showed that the population abundance of endophytic bacteria contained in the healthy banana plants and the infected plants was different. The most abundant population was found in roots , the middle in the bulb tissue and the least in leaves; The abundance of endophytic bacteria increased after infection with FOC; And most of the sequences were phyl0genetically close to Cyanobacteria, Chloroflexi, Actinobacte- ria, Proteobacterium, Bacteroidetes, Firmicutes and Chlorobi ; But the homology for one of the sequences was only 91%. It may represent a new taxon.
Keywords：BananaEntophytic bacteriaDenaturing gradient gel electrophoresis （DGGE）Community structureDiversity
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)