In vitro embryo culture and tetraploid induction of Hawthorn
Abstract：In vitro culture of immature and mature embryos and tetraploid induction of hawthorn were investigated. The rate forming seedlings from in vitro immature embryo of hawthorn harvested 40 days after blooming was very low and it was less than 20% for 15 tested accessions. But the rate forming seedlings from in vitro mature embryo was high, and it was more than 50% for 4 accessions. The mature embryos of hawthorn was dipped in the mixed solution of 0.5% colchicine and 1% dimethyl sulfoxide for 48 h and transferred to SC solid medium supplemented with 0.1mg·L^-1 IBA and 1.0 mg·L^-1 TDZ to induce bud dif- ferentiation. Cytological analysis showed that 3 out of 67 regenerated plants of Longhuafenrou were tetraploid and the variation rate reached 4.5%. The system of in vitro induction of tetraploid for hawthorn was developed, and this laid a sound foundation for breeding of polyploid cultivars in hawthorn.
Keywords：HawthornEmbryoIn vitro cultureTetraploid
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)