Cloning and real-time fluorescence quantitative expression of SFB genes from apricot cultivars in Xinjiang area
Abstract：The pollens from 27 apricot cultivars in Xinjiang area have been used to clone and sequence the SFB gene in transcription levels. The pollens of Dongxing, Suluke, Kaertuoyong were cultured in Vitro and the expression characteristics of SFB gene in different periods were analyzed. The results showed that four different size fragments were cloned from 27 apricot cuhivars. The fragments were cloned from 26 cuhivars by primer combination 1.The fragments of Beixinnaer and Saimaiti were 447 bp. The fragments of Dayou- jia were 446 bp, and the others were 434 bp. The fragment which was 660 bp was cloned from Tuohuti by primer combination 2. Sequence alignment showed the SFB genes of 27 cultivars were 20 sequences. The sequence alignment with other cultivars showed that the 20 sequences were new SFB sequence, the acces- sion number of GenBank were HQ148064-HQ148083. SFB genes in pollen of 3 apricot cultivars were ex- pressed during the 4 different culture period, the transcript abundance showed increase at first and then decrease, the transcript abundance at the stage of 24 h in vitro culture was highest in all cuhivars, howev- er, the transcript peak abundance of 3 cultivars were different.
Keywords：ApricotSelf-incompatibilitySFB geneRTFQ PCR （Real Time Fluorescence Quantitative PCR）
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)