Molecular identification of peach bud sports from Beijing 28 with ISSR and SSR

Abstract：Molecular identification is important for the protection of new varieties and studies of germplasm resources. Of fingerprinting techniques, Inter-Simple Sequence Repeat （ISSR） and Simple Sequence Re- peat （SSR） were among the most informative and powerful. In this study, we identified a peach mutation cuhivar from Beijing 28 by ISSR and SSR based on the identification of phenotypic characters. The results showed that different variations of phenophase and fruit characters were observed in bud sports. The SSR primers could produce one or two discrete fragments in these accessions, and polymorphic bands were found between C2 and other samples in CPPCT022 and BPPCT023. Thirty nine ISSR primers amplified 314 bands, 36 bands were polymorphic, and the polymorphic percentage was 11.46%. The genetic simi- larity coefficient （GS） estimated from these molecular data ranged from 0.91 to 0.99, with an average of 0.95. The GS of three mutation accessions was 0.99 and of two controls from same orchard was 0.96. The combined grouping association indicated that these mutation accessions and two controls from the same or- chard were clustered distinctly, and the genetic relationship was most closely between C2 and mutations. The three bud sports should be most likely from the control C2 combined the identification of phenotypic variation, and CPPCT022 and BPPCT023 might be the variation sites. These results provided valuable references for identification and protection of a new variety, classification and application of peach germplasm in commercial nurseries.