Cloning and transcriptional analysis of resistance gene Fom-2 from Cucumis melo ssp. Agrestis Jeffrey
Abstract：Fusarium wilt （Fusarium oxysporum f. sp. melonis Snyder and Hansen） is one of destructive disease in melon crop production. Fore-2 gene was genetically identified to control the resistance of races 0 and 1. Resistance to fusarium wilt breeding is one of important targets for melon breeding. In this study, we cloned the Fore-2 gene from Baizhuogua melon （Cucumis melo ssp. agrestis Jeffrey） by using homo- logical cloning method, which was deduced to encode 1099 amino acids and exhibited high similarity to NBS-LRR type of R genes in plant. The deduced FOM-2 protein contained NB-ARC and SCOP typical motifs of R gene. The expression of Fore-2 gene was highly induced after inoculation with fore race 1. In eonclusion, the cloning of Fore-2 gene is good for breeding with resistance to fusarium wilt.
Keywords：Cucumis meloBaizhuoguaFusarium wiltFom-2 gene
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)