Establishment of SSR fingerprinting database for 38 cultivars of straw- berry （Fragaria ananassa） native to Europe and America
Abstract：The total DNA of 38 strawberry cultivars originating in Europe and America , such as Earliglow, Induka, Darselect ,Senga, Litessa, were amplified by 44 pairs of SSR primers. The PCR products were separated in 8% polyacrylamide sequencing gels. Some factors which affected amplified products, such as dNTPs, Taq DNA polymerase,primer and so on were also studied. Optimum condition was as follows：25 μL PCR reaction system consisted of 1 xPCR Buffer, 2.5 mmol ·L^-1 MgCl2, 0.4 μmol primer, 0.2 mmol dNTP, 1.5 U Taq polymerase and 60 ng DNA. The amplification conditions were 94 ℃ for 1 rain, 35 cy- cles of 94 ℃ for 30 s,annealing temperature for 30 s ,72 ℃ for 30 s and a final extension step at 72 ℃ for 7 rain. Ten pairs of primers which could amplify stable and distinct band were selected. The electrophoresis results of the 38 strawberry DNA samples were evaluated and analyzed. Eventually, 29 cuhivars could be distinguished by the fingerprint map constructed by only 4 pairs of SSR primers.
Keywords：StrawberryFingerprintingMolecular markerSSR
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)