Isolation and functional analysis of a elongation factor 1A（MaEF1A） gene from banana
Abstract：A cDNA sequence with length of 1 341 bp named MaEF1A had been obtained by screening postharvest banana fruit cDNA library.The results of bioinformatics analysis showed that MaEF1A shared 84% and 83% homologous with Lilium longiflorum Thumb and tobacco,respectively,which was suggested to be a banana elongation factor 1 alhpa（EF 1A）.MaEF1A contained an integrated ORF and three conserved regions of GTP-EFTU,GTP-EFTU-02 and GTP-EFTU-03.The expressions of MaEF1A in different banana tissues and fruit in different postperiod was investigated by RT-PCR.The result indicated that MaEF1A was differently expressed in different banana tissues and different ripening stages of postharvest fruits.The expression levels in root and flower were higher than in stems and leaves.The expression trend of the gene was slowly increased from 0 d to 18 d and reached the peak at 20 d and then decreased,which was similar to ethylene release during postharvest ripening of banana fruits.
Keywords：BananaFruit cDNA libraryMaEF1ART-PCR
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)