Analysis of genetic relationship among 20 Vitis germplasm resources by SSR markers

Abstract：Twenty Vitis germplasm resources were used as materials for analyzing their genome polymorphism by SSR markers in the present study.Nineteen pairs of polymorphic primers selected from 245 pairs were used for SSR amplification.144 DNA fragments were generated and all of them were polymorphic bands（the percentage of polymorphic band,PPB=100%）.Jaccard similarity coefficients of the SSR amplification results were analyzed by NTSYSpc2.10e software and the genetic similarity coefficient was 0.630 to 0.900 with the average genetic similarity coefficient of 0.765.The clustering dendrogram constructed by UPGMA method indicated that 20 Vitis materials could be divided into four major groups at the similarity coefficient of 0.665.The first group included V.vinifera,American grape hybrids and Euro-American hybrids.The second group included V.davidii Foёx.V.quinquangularis Rehd.,V.piasezkii Maxim.,V.hancockii Hance,V.thunbergii Sieb..Zucc.and V.pseudoreticulata W.T.Wang.The third group included the hybrids（V.amurensis×V.vinifera） ×V.amurensis.The fourth group included V.amurensis Rupr.materials.The results suggested that the genetic correlations were much lower between V.amuensis and V.vinifera,or Euro-American hybrid,but higher between V.vinifera and Euro-American hybrid,or American hybrids.