Identification of skin colour variant cultivars（strains） in pear by AFLP and SRAP markers

Abstract：In order to reveal the genetic difference between the skin colour mutants and their original varieties,molecular identification and analysis were conducted on three groups of skin colour variations in pear by AFLP and SRAP makers.A total of 1 089 gene loci were screened by 54 AFLP primer-pairs,of which 688 were polymorphic with the polymorphism percentage of 63.2%.And 210 gene loci were screened by 20 SRAP primer pairs,of which 76 were polymorphic with the polymorphism percentage of 36.2%.Results showed that seven AFLP and three SRAP primer pairs could distinguish Nanguo and red variant strain;three AFLP and one SRAP primer pairs could identify the difference among Doyenne du Comice,Red Doyenne du Comice,Early Red Doyenne du Comice,and green variant strain of Early Red Doyenne du Comice;Red D Anjou and green variant strain could not be distinguished by any AFLP and SRAP primer pairs.Combining with data revealed by AFLP and SRAP,specific patterns of 4 cultivars or strains were constructed.All cultivars or strains,except Red D＇Anjou and its variant strain,could be identified by SRAP primer em11/me5,and this primer could be used as a useful marker in molecular identification.