Genetic relationship analysis of Fortunella germplasm resources from China and Vietnam by ISSR markers
Abstract：In order to make good use and innovate germplasm resources of kumquat,the genetic relationships of 25 Fortunella Swingle germplasm resources from China and Vietnam and the variation accessions of F.crassifolia Swingle were examined by ISSR markers in this study.Of the 100 ISSR primers screened,only 10 primers gave reproducible,polymorphic DNA amplification patterns with a total of 120 amplified bands.The percentage of polymorphic band was 85%.By banding patterns obtained from these 10 primers,each accession could be distinguished from the others.The genetic similarity ranged from 0.48 to 0.99 UPGMA relationship tree was established based on the similarity coefficients.At the coefficient level of 0.85,the 25 germplasm resources were divided into 8 groups.The classification results were discussed in this paper.Shanju（Fortunella Swingle） found along the border between China and Vietnam could be a new variety or species of Fortunella Swingle.
Keywords：Fortunella SwingleGermplasm resourcesISSRGenetic relationshipVariation of F.crassifolia Swingle
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)