SSR analysis on genetic diversity of wild Chinese chestnut populations
HUANG Wu-gang
CHENG Li-li
ZHOU Zhi-jun
LIU Jian-li
Abstract：Ten SSR loci isolated from Castanea mollissima Blume were used to evaluate genetic diversity of the wild Chinese chestnut. 84 alleles were successfully amplified from 69 accessions of the four wild populations. With an average level of 8.4,the numbers of alleles per locus ranged from 4 to 13. Average values of Na and effective Ne of alleles were 8.4 and 4.998,respectively. Average values of H_e (mean expected heterozygosity)and PIC (polymorphism informative content) were up to 0.777 and 0.739,respectively. However,the highest genetic diversity was found in the Shaanxi population. The low genetic differentiation (G_(st) = 0.141)showed that the genetic variation mainly occurred within the populations. UPGMA phenogram showed that the populations of Yunnan,Chongqing and Anhui province were divided into one of the two groups, and that principal coordinate analysis also presented the same result. Two methods showed that no significant correlation between the genetic relationships and geographical distance was revealed among the populations.
Keywords：Wild Chinese chestnutSimple sequence repeat(SSR)Genetic diversity
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 227-232 )