Cloning and expression analysis of APETALA2 gene from grapevine (Vitis vinifera)based on EST database
WANG Chen
LIU Hong
FANG Jing-gui
SONG Chang-nian
CAO Xue
YANG Guang
ZHANG Zhen
Abstract：APETALA2 (AP2)plays an important role in Arabidopsis development,especially in the development of flower organs. Based on the relative conservation of plant homologous genes, a full-length Vitis vinifera homologue of AP2 (Vv-AP2)was bioinformatically cloned in this study. Accordingly,the 5'- and 3'-end sequences were obtained from cDNA of flower of Xiang yue grapevine cultivar by 3'RACE and specific PCR with two gene-specific primers designed against the Vv-AP2 sequence,based on the full length cDNA of Vv-AP2 (2 208 bp) which was spliced. This Vv-AP2 cDNA included an open reading frame (ORF)of 1 536 nucleotides, 5'-untranslated region (UTR) of 268 bp, and 3'-UTR of 376 bp. The 3'-UTR contains a Poly~+(A) of 28 bp. The sequence has been deposited in GenBank database with the accession number of FJ809943. The deduced amino acid sequence of Vv-AP2 includes 551 amino acids,contains a putative nuclear localization signal sequence (KKSR) and two highly conserved AP2 domains. The semi-quantitative RT-PCR and SYBR Green I Real-time qRT-PCR were employed to analyze the expression of Vv-AP2 in different organs of grapevine and the result showed that Vv-AP2 is expressed in much higher level in inflorescence and flower than that in leaf and stem.
Keywords：GrapevineVv-AP2Gene cloningExpression
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 207-212 )