Studies on somatic embryogenesis and plant regeneration from anthers of Guangxi-2, a line of Chinese wild grape (Vitis pseudoreticulata)
ZHI Yu-xi
ZHANG Jian-xia
WANG Yue-jin
Abstract：In order to offer a regeneration system for functional characterization of resistant genes in grapevine genetic trans-formation, anthers from powdery mildew-susceptible line Guangxi-2 of Chinese wild Vitis pseudoreticulata were used to in-duce somatic embryos and establish regeneration system. The induction of secondary embryos and the normal seedling from abnormal germinated somatic embryo were also explored in this study. The results indicated Guangxi-2 anthers could be in-duced and formed embryo callus on B_5 medium containing 6-BA 4.0 mg·L~(-1)+ 2, 4-D 0.7 mg·L~(-1)+ CH 1.0 g·L~(-1)+PVP 1.0 g·L~(-1)+sucrose 30.0 g·L~(-1) after 3-month subculture. The B_5 medium containing 2, 4-D 0.7 mg·L~(-1)+ CH 1.0 g·L~(-1)+ PVP 1.0 g·L~(-1)+sucrose 15.0 g·L~(-1) was beneficial to form embryos. The germination of embryos on thei/2 B_5 liquid medium containing CH1.0 g·L~(-1)+PVP 1.0 g·L~(-1)+sucrose 15.0 g·L~(-1) was 14 days earlier than on the B_5 solid medium. The medium for root formation on the germinated embryo was W PM medium containing IBA 0.15 mg·L~(-1)+ A C 0.5 g·L~(-1)+ sucrose 30.0 g·L~(-1). The MS medium containing 6-BA 1.5 mg·L~(-1)+ IBA 0.2 mg·L~(-1)+ sucrose 30.0 g·L~(-1) was favorable to the normal seedling formation from mal-formed embryos. The embryos in early developmental stage could be generated secondary somatic embryos on the B_5 medium containing 6-BA 1.0 mg·L~(-1)+2,4-D 0.5 mg·L~(-1)+ CH 1.0 g·L~(-1)+ PVP 1.0 g·L~(-1)+ sucrose 30.0 g·L~(-1).
Keywords：Chinese wild VitisVitis pseudoreticulataAntherSomatic embryo inductionRegeneration plant
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 18-23,封3 )