Meiotic observations on pollen mother cells of sexual progenies of citrus somatic hybrid
GAO Juan
YI Hua-lin
HU Zhi-yong
Abstract：The sexual progenies of citrus somatic hybrid possess three parents' genetic materials. In order to investigate their cytological behavior,the sexual progenies of a cross between allotetraploid hybrid [Hamlin sweet orange (Citrus sinensis Os-back) +Rough Lemon (C. jamb hiri Luss)] (as the pollen parent) and mono-embryonic diploid type Iben No. 4 [Huanongben-dizao tangerine (C. reticulata Blanco)×Ichang papeda (C. ichangensis Swingh)] was used as materials for observing the mei-otic course of their pollen mother ceils. The results showed that the PMC of diploid progenies showed normal meiosis,while a series of abnormal meiotic phenomena were observed in triploid progenies, such as univalent (56.9%)and muhi-valent, one to three chromosomes beside the equatorial plate at metaphase Ⅰ (51.2%)or metaphase Ⅱ (38.7%). Chromosome bridges, chro-mosomes fragment,lagging chromosomes at anaphase Ⅰ or anaphase Ⅱ and the small nucleus in tetrads were also frequently observed. Meanwhile, the relationship between the meiotic stage and diameter of floar bud was analyzed.
Keywords：Citrus somatic hybridSexual progenyMeiosisChromosome
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 8-12 )