Molecular cloning,characterization and expression of iron superoxide dismutase gene from Ginkgo biloba
LI Lin-ling
CHENG Hua
XU Feng
WANG Yan
CHANG Jie
CHENG Shui-yuan
Abstract：The full-length cDNA sequence of Ginkgo biloba L. iron superoxide dismutase gene (GenBank: FJ555019) was isolated from Ginkgo biloba. The full-length cDNA of GbFeSOD contained a 720 bp open reading frame (ORF). The 3D structure modeling showed that GbFeSOD contained 11 helixes and 3 sheets and had a basketry motif in the enzyme core. Phylogenetic tree analysis revealed that GbFeSOD shared the same ancestor with other FeSOD. The result of Southern analy-sis showed that Gb FeSOD gene was encoded with a small gene family in Ginkgo biloba Northern hybridization analysis showed that GbFeSOD had the highest expression in leaves,then in stem ,and the least in fruit. There was no expression found in roots. The expression of GbFeSOD could be induced by ABA,osmotic stress, low temperature and heat injury; among them ,levels of FeSOD transcripts increased placidly a few hours after chilling stress at 4℃. Expression of the FeSOD gene was enhanced dramatically by heat stress at 40 ℃. However,the expression of GbFeSOD first increased and then decreasedafter heat stress at 44 ℃.
Keywords：Ginkgo biloba L.Iron superoxide dismutaseExpression analysis
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 840-846 )