Anthocyanin accumulation and UFGT gene expression analysis by RT-PCR from cultivars belonging to Vitis vinifera
CHENG Jian-hui
LEI Ming
YANG Fue-hen
WU Yong-hua
WU Jiang
Abstract：Anthocyanin accumulation and coloration in fruit of Vitis vinifera L.grown in plastic-greenhouse was studied un-der the local natural conditions.The results showed that anthocyanin in fruit of cuhivars of V.vinifera L.started to accumulate at veraison stage, so that the fruit color started becoming from green to red till maturity.Anthocyanin components in fruit of V.vinifera L.were mainly of mono-glucoside , which was different from the cuhivars derived from the cross between V.vinifera and Vitis labrusca.In addition,there was also difference in anthocyanin components between cultivars of V.vinifera L.Ap-plication of exogenous abscisic acid and rahmnose under greenhouse or in in vitio condition could promote anthocyanin accu-mulation in berries of Red Globe cultivar and Pn-3-O-glucoside content in fruit peel was increased remarkably, while the sucrose was reverse.Semi-quantitative RT-PCR analysis showed that the glucose flavanone transferase RGufgt gene (Gen-Bank: DQ513314)was expressed in young leaf, not expressed in young green fruit and highly expressed from fruit verasion stage to maturity.The process of RGufgt gene expression was completely consistent with the anthocyanin accumulation.
Keywords：Grape(Vitis vinifera L.)AnthocyaninAccumulationUFGTgeneRT-PCR
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 808-812 )