Factors affecting regeneration of embryonic axis of peach
WAN Chun-yan
CONG Fang
HAN Ming-yu
ZHAO Cai-ping
LIU Hang-kong
TIAN Yu-ming
Abstract：Experiment was conducted with Shuguang, Tiantaowang and Shahong (Amygdalus persical L.) for studying the effects of different elements in medium, cut place and subculture time on inducing callus and regeneration of adventitious shoots of embryonic axis.Results showed: (1)The best medium for both callus inducing and adventitious shoot regeneration from emhryonic axis of shucuang,Tiantaowang and Shahong was same; For Shuguang and Tiantaowang on medium of LP+TDZ 3.0 mg·L~(-1)+BA 1.0 mg·L~(-1)NAA 1.0 mg·L~(-1), the ratio of their callus was 100％, the ratio of adventitious shoots was 24.44％ and 55.56％ respectively; for Shahong on medium of MS+TDZ 4.0 mg. L~-1BA 1.0 rag. L~(-1)NAA0. 1 mg· L~(-1), the ratio of callus reached 100%, while the ratio of adventitious shoots was 46.67%.(2) The adventitious shoots regenerated from near cotyledon end were significantly more than that from far end of these three cultivars,their average adventitious buds regener-ated were 6.33, 9.67 and 8.00.(3) Along with the subculture times increasing,there appeared great difference on the mor-phology of callus, and the ability of regeneration of adventitious shoots was obviously different as well.
Keywords：Peach A mygdalus persical L.Embryonic axisCallusAdventitious shoots
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 804-807,封底 )