Studies on fingerprinting of seedless triploid watermelon cultivars and hybrid purity identification by SRAP markers
ZHAO Sheng-jie
LIU Wen-ge
YAN Zhi-hong
HE Nan
Abstract：SRAP (Sequence-Related Amplified Polymorphism) marker was applied to analysis DNA fingerprinting of 9 triploid seedless watermelon cultivars and identify hybrid purity. The results showed that 24 primer pairs selected from 30 amplified 109 polymorphie bands with an average of 4.5 polymorphie bands per primer pairs and the polymorphic rate of primer pairs was 12.5% to 80%. All 9 cultivars could be distinguished from each other combining several SRAP primer pairs. In the fingerprinting analysis of Heimi No.5 and its parents, specific bands were generated on Heimi No.5 with ME4/EM3 primer pairs and enabled to distinguish the female parent from the F, hybrid. This primer pair was certified to be suitable for purity identification of Heimi No.5 in experiments.
Keywords：Seedless watermelonTriploidDNA fingerprintingPolymorphicSRAP
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 687-691 )