Study on the difference in expression profiles of microRNA among different organs of strawberry
LI He
YIN Dong-sheng
WANG Zhi-gang
HUANG Fei-fei
CHANG Lin-lin
ZHANG Zhi-hong
Abstract：MicroRNA (miRNA) plays an important role in plant growth and development. Using total RNA extracted by CTAB method as templates, the expression profiles of 6 miRNA in root, receptacle, shoot tip and leaf of strawberry were studied by real-time RT-PCR through stem-loop RT primers. When the relative quantity of 6 miRNA in strawberry leaf was as 1, the result of different expression showed that the highest expression in receptacle was miR164, reaching 6.615, obviously higher than the quantity in root (0.485), shoot tip (0.371) and leaf (1). While the expression of miR172 in shoot tip and receptacle were 0.029 and 0.020, lower than that in leaf. The lowest expression of miR172 was in root, only 0.000 845 4, the same as miR167, only 0.053. No obvious difference in expressions of miRi56, miR159 and miR165 was found in 4 kinds of strawberry organs.
Keywords：StrawberryMicroRNAReal time RT-PCRExpressionOrgan
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 632-637 )