Adventitious bud regeneration in vitro from leaves of peach cultivars
CONG Fang
HAN Ming-yu
ZHAO Cai-ping
WAN Chun-yan
LIU Hang-kong
Abstract：Experiment was conducted with Shuguang, Babygold 5 and Tiantaowang peach cultivars for studying the effects of the basic media, different plant growth regulators, different genotypes and subculture times on the regeneration of leaves in vitro. Among the 3 cuhivars, Babygold 5 and Shuguang showed strong regeneration capacity. Results showed that the suitable basic medium and plant growth regulator was LP+ 1.5 mg· L~(-1) TDZ+0.15 mg· L~(-1) NAA in dark cultured period. After 3 weeks of light treatment Babygold 5 and Shuguang cultured on LP+0.5 mg· L~(-1) TDZ+0.3 mg·L~(-1) KT+0.3 mg·L~(-1) NAA and LP+0.6 mg·L~(-1) TDZ+0.3 mg·L~(-1) KT+0.4 mg·L~(-1)2, 4-D grew thick buds, their regeneration frequency were 21.5% and 14.6%. The leaves of Shuguang and Babygold 5 collected from the 1st to 11th regeneration produced much higher rate of callus than those of the former 3 generations of embryos. The rooting rate of the Shuguang regeneration was 100% and the mean number of roots was 7.9 in the medium of 1/2MS +0.5 mg· L~(-1) NAA.
Keywords：PeachLeaves in vitroRegenerationAdventitious bud
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 614-618,封4 )