Organogenesis, somatic embryogenesis and plantlet regeneration from leaf explants of Actinidia kolomikta cultured in vitro
LAN Da-wei
LIU Yong-li
Takashi Harada
Abstract：Leaf explants from Actinidia kolomikta were cultured on BW media containing 30 g/L sucrose and 2 g/L Gelrite and supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Zeatin. Adventitious buds were generated from calli derived from leaf explants and developed into shoots. Shoot regeneration rate and the number of shoots regenerated increased with the increase in Zeatin concentration and decreased with the increase in 2,4-D concentration. Embryogenesis was achieved in the BW medium added with 1 μmol/L each of 2,4-D and Zeatin. Subculture of the embryogenic callus led to secondary embryogenesis with a high frequency of 66.7％. In rooting culture of shoots, roots formed directly from the basal portion of shoots on the medium containing 2,4-D or α-Naphthaleneacetic acid (NAA). Plants were successfully acclimatized to the field conditions with a survival rate of 100％.
Keywords：Actinidia kolomiktaAdventitious budsSomatic embryos24-DZeatin
Publication Date：2007-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 218-221 )