Construction and study of a vector for antisense expression of acid invertase gene from melon
YU Xi-yan
TIAN Hong-mei
FAN Ji-de
WANG Xiu-feng
Abstract：Improvement of fruit quality is an important object in melon breeding. Application of antisense RNA strategy to reduce acid invertase activity provides a new feasible method for breeding melon cultivars with high sucrose accumulation. In a previous study we cloned a cDNA fragment of acid invertase from melon fruit into pMD18-T. For constructing antisense melon acid invertase gene vector (Anti-MAI1), the gene was digested with BamH Ⅰ and Hinc Ⅱ to release a fragment with 1038 bp coding region,which was then inserted between the cloning sites of BamH Ⅰ and Sma Ⅰ of pROK2. Anti-MAI1 was transferred into tobacco mediated by Agrobacterium tumefacions LBA4404. PCR and PCR-Southern blot were performed,and the results showed that MAI1 had been transformed into the genomic DNA of tobacco.
Keywords：MelonAcid invertase geneAntisense expression vectorTobaccoTransformation
Publication Date：2006-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 850-853 )