Somatic embryogenesis and plantlet regeneration in Citrullus lanatus cv. Zhengkang No.4
NIU Shan-shan
SONG Shang-wei
YAN Feng
MIAO Hong-xia
Abstract：Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3 d, then exposed to a photoperiod of 16/8 (lignt/dark) h/d for 3 d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA (2.0 mg/L), kinetin (1.0 mg/L)and GA3 (1.0 mg/L). The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8 h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures. The optimum rooting medium was 1/2 MS+IBA (0.3 mg/L). The highest induction rate of somatic embryo was 25%. Embryogenic calli developed into mature somatic embryos and plantlets.
Keywords：Citrullus lanatusSomatic embryogenesisPlantlet regeneration
Publication Date：2006-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 406-410 )