Organ formation and plant regeneration in vitro of Actinidia polygama
Liu Yong-li
LAN Da-wei
BI Jing-hua
CHEN Chao
Takashi Harada
Abstract：Petiole explants collected from Actinidiapolygama were cultured on BW media (a combined medium of half strengths of both broad-leafed tree medium and woody plant medium) containing 30 g/L sucrose and 8 g/L agar, and 12combinations of IBA (0, 0.01, 0.1 or 10 μmol/L) and CPPU (0, 0.1 or 1 μmol/L). Callus formation was promoted by increasing CPPU and IBA concentrations. Adventitious buds formed from the outer layer of callus that derived from the petiole explants from A. polygama. Shoot formation was increased with the increase of CPPU concentration and decreased with the increase of IBA concentration. When the shoots were transferred to BW medium supplemented with 1 μmol/L NAA alone,the intact shoots showed a 100% rooting rate after 40 days, and grew very healthily.
Keywords：ActinidiapolygamaAdventitious budShootCPPUIBA
Publication Date：2005-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 220-223 )