Effect of Freezing Solution on Cryopreservation of Lateral Buds of Wild Tara Vine (Actinidia arguta)
Abstract：Lateral buds of tara vine (A ctinidia arguta Planch.) were collected in late December and surface-sterilized with ethanol, followed by sodium hypochlorite solution. Effects of the situation of freezing solution in different composition, temperature and immersing duration were investigated. The optimum concentration of DMSO for cryopreservation of the buds was 15%, and the optimum temperature of freezing solution was 0℃. The buds immersed in liquid nitrogen after pre-freezing of freezing to -30℃ showed the highest survival rates at high concentrations (8 mol/L and 11.5 mol/L) of glycerol. The buds loaded with glycerol before immersing in freezing solution gave the highest survival rate with a loading solution containing higher concentrations of glycerol. The highest survival rates were obtained with lateral buds immersed in the freezing solution for 0, 0.5 and 1 hours.
Keywords：CryopreservationTara vineDMSOGlycerol
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 266-269 )