Localization of Ca2+ by Antimonite Precipitation Technique and Its Appli cation to Pear Physiological Research
Peng Sbu'ang
Abstract：The method of localising Ca2+ by antimonite precipitation technique,the detailed procedure and effects of its application to fruit physiological research were presented in this paper.The results showed that with treatment of the fixative solution added with antimonite,the free Ca2+ in pear different organs and tissues could be changed into antimonite calcium and precipitated in situ,which,after being stained,appeared as black particles under electronic microscope,whereas such particles could not be observed in the treatment without antimonite.Furthermore,the samples were treated with EGTA,a specific reagent,in order to see whether the black particles would disappear and to confirm that the black particles were Ca2+ so that Ca2+ could be localised and marked.In different tissues and during different physiological periods of the same tissue the density and site of the marked Ca2+ were different,which could be used to study the mechanism of Ca2+ by means of its fluctuation.The problems,to which we should pay attention when the localization of Ca2+ was conducted by this method,were also discussed.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 140-144 )