The Determination of Carotenoids in Apple Peel by HPLC
Abstract：A reversed-phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C18 column(4.6 mm×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β-carotene were 94.27%～99.82%,84.56%～89.41%，87.53%～93.54%，and 88.08%～93.24%.‘Fuji’ apple contained zeaxanthin (5.9×10－3 μg/mm2) and β-carotene (5.4×10－3 μg/mm2) and Red Delicious apple only zeaxanthin (9.1×10－3 μg/mm2).
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 95-97 )