A metabolomics study on the impact of noise exposure on red blood cell metabolism in mice
WANG Kai
GUO Baoshi
CHE Dandan
WANG Quan
HE Qican
QU Mingyue
CHEN Gan
Abstract：Objective To investigate the impact of noise exposure on red blood cell metabolism using metabolomics technology,and to provide a reference for further research and exploration of the mechanisms underlying the impact of noise exposure on human health.Methods Eight male C57BL/6J mice were selected to establish a noise exposure model,and were subjected to noise stimulation at an intensity of 90-105 dB.Additionally,6 mice were chosen as the control group and housed in a box of the same specification but without additional sound output.Changes in body weight were monitored,and the plasma amylase,cholesterol,urea nitrogen,and creatine kinase were detected.Metabolomics analysis was performed to detect changes in red blood cell metabolism.Results After 10 consecutive days of noise exposure,the body weight of the exposed group decreased by 17.8%compared to the control group[(19.7±1.8)g vs.(23.9±1.9)g,t=4.317,P＜0.01].Compared with the control group,the noise-exposed group showed markedly elevated plasma levels of amylase[(917.6±86.1)U/L vs.(719.8±90.4)U/L,t=3.690,P＜0.01],cholesterol[(3.9±0.4)mmol/L vs.(3.1±0.4)mmol/L,t=3.569,P＜0.01],and urea nitrogen[(8.7±1.1)mmol/L vs.(6.2±1.0)mmol/L,t=3.698,P＜0.01],whereas the level of creatine kinase was significantly decreased[(877.8±383.3)U/L vs.(1 568.3±163.4)U/L,t=4.029,P＜0.01].Metabolomics analysis revealed that noise exposure led to significant decreases in erythrocyte sphingosine-1-phosphate and 6-hydroxymelatonin levels.These changes were associated with the down-regulation of multiple signaling pathways,including the Fcγ receptor-mediated phagocytosis,Apelin signaling pathway,phospholipase D signaling pathway,and calcium ion signaling pathway.Conclusions Short-term noise exposure can lead to a decrease in body weight and changes in red blood cell metabolism in mice,involving red blood cell metabolic pathways such as Fcγ receptor-mediated phagocytosis,Apelin signaling pathway,and calcium ion signaling pathway.This study provides new insights and experimental evidence for the prevention and management of non-specific damage caused by noise pollution.
Keywords：noise exposurered blood cellsmetabolomicsApelin signaling pathwaycalcium ion signaling pathway
Publication Date：2025-10-28
Online Publishing Date：2025-11-24(First online date of this platform, not the publication date of the document)
Pages：6( 358-363 )