Study on the correlation between Mycoplasma Hominis and Ureaplasma Urealyticum infection and infertility patients and drug sensitivity analysis

Liu Jiajing; Zhang Yubing

doi:10.3969/j.issn.1672-8521.2025.01.005

Abstract：Objective:To explore the correlation between mycoplasma hominis(MH)and ureaplasma UU infection and infertility patients and the results of drug sensitivity analysis.Methods:This was a prospective study.A total of 116 women with infertility who received treatment in Nanping People's Hospital of Fujian from June 2021 to July 2022 were selected as the study subjects(the observation group).And another 130 women who underwent physical examination in the same hospital during the same period were selected as the healthy control group.Cervical secretions of the subjects in the two groups were respectively detected for MH and UU.The drug sensitivity test was carried out at the same time,and the test results of the two groups were compared.Result:Among the patients included in this study,there were 54 cases of primary infertility and 62 cases of secondary infertility.The detection rates of MH,UU and UU+MH in both primary and secondary infertility patients were higher than those in the healthy control group(P＜0.05),but there was no statistical significance between secondary and primary infertility patients(P＞0.05).The results of drug sensitivity analysis showed that UU had the highest drug sensitivity to tetracycline and doxycycline(91.30％and 89.13％,respectively),and higher sensitivity to ofloxacin,lucamycin,azithromycin and levofloxacin(82.61％,82.61％,78.26％and 76.09％,respectively).The sensitivity rate to clarithromycin,roxithromycin,miamycin and sparfloxacin was generally(52.17％,50.00％,47.83％and 47.83％,respectively).The drug resistance to ciprofloxacin in fluoroquinolones was high,with a sensitivity rate of 15.22％.MH was sensitive to doxycycline,lucamycin and melomycin(84.62％,80.77％and 76.92％,respectively),while the sensitivity rate to clarithromycin,sparfloxacin,ofloxacin and levofloxacin were generally(69.23％,38.46％,34.62％and 34.62％,respectively).The resistance to tetracycline,azithromycin,erythromycin,roxithromycin and ciprofloxacin was high,and the sensitivity rates were 7.69％,7.69％,7.69％,3.85％and 3.85％,respectively.The drug with the highest sensitivity to MH+UU mixed infection was tetracycline,with a sensitivity of 100％,followed by doxycycline and doxamycin,with a sensitivity of 81.82％and 72.73％,respectively.other drugs included in this study showed very high resistance.According to the statistics of drug resistance of 83 mycoplasma strains,there were 3 sensitive UU strains,and no drug-resistant strains were found.The resistance of UU to 2 and 3 antimicrobial was relatively high,which was 17.39％and 21.74％,respectively,and the resistance of UU to 4 antimicrobial was higher,which was 28.26％.The resistance of MH to 3 and 4 antimicrobial agents was 19.23％and 26.92％,respectively.The resistance rate of MH+UU mixed infection to 9 and 10 antibiotics was the highest(45.45％and 54.55％,respectively),while the resistance to 11 antibiotics was low(9.09％).Conclusions:Mycoplasma hominis and ureaplasma urealyticum are closely associated with infertility.Routine testing should include the detection of these two infections,and sensitive antibiotics should be selected for treatment according to the results of drug sensitivity test.