Establishment of stable pathological scar models

Jiao Ya; Qi Yongjun; Gong Hongmin; Wang Xiao; Jiang Duyin

doi:10.3969/j.issn.1672-8521.2017.01.004

Abstract：Objective:To compare the stability of two pathological scar models,nude mice model and rabbit ears model,and improve the modeling method for selection of the appropriate animal models used in pathological scar research.Methods:Human hypertrophic scar and keloid with or without epidermis were transplanted into the dorsa of nude mice subcutaneously to establish the nude mice scar models.The method of replicating mice model by transplantation with epidermis was improved by cutting off the skin on the top of the transplanted tissue a week after transplantation,while scar epidermis was removed before transplantation in the mice transplanted without epidermis.Rabbit's ears scar models were made by three methods respectively:removing the perichondrium and cartilage,retaining the perichondrium and cartilage,removing the perichondrium but retaining cartilage.Then the quality of the models was assessed by observing the effects of scar formation,stability time and pathological alteration of scar models.Results:The nude mice models showed pathophysiologic characteristics of hypertrophic scar or keloid quickly and steadily.The stability time was longer (15 and 20 weeks respectively in hypertrophic scar group or keloid group transplanted with epidermis,8 and 10 weeks respectively in hypertrophic scar group or keloid group transplanted without epidermis),and the degree of scar hyperplasia was more clearly in the mice modeltransplanted with epidermis.The rabbit's ears models revealed a complete set of development process of hypertrophic scar.In the group of removing the perichondrium but retaining cartilage,the stability time (15 weeks) was longer and hypertrophic degree was stronger obviously than the other two groups (9 or 10 weeks of stability time).Conclusions:For construction of nude mice models of scar hyperplasia,the method of remaining epidermis is better,and removing the perichondrium but retaining cartilage of rabbit's ears is the best choice for establishment of rabbit's ear scar model.