Effect of Xuebijing injection (血必净注射液)on immune function of T lymphocytes in systemic lupus erythematosus in mice
Wang Yanbo
Zhao Yanqin
Ding Yanlan
Wang Qiang
Abstract：Objective:To observe the effect of Xuebijing injection (血必净注射液)on T lymphocyte and its potential mechanisms in systemic lupus erythematosus (SLE).Methods:A widely used mouse model,SLE-prone BLLF1 mice aged 8-10 weeks,was em-ployed. Xuebij ing inj ection in a dose of 6.4 ml/kg was given via the intraperitoneal administration in the Xuebij ing treatment group. Spleen sample was divided as following:one part was used to procure DC with MACS microbeads and T cell by using col-umn of nylon wool,and the other part was used to determine gene and protein expressions of various cytokines. Cells were cul-tured and the contents of cytokines released into supernatants were determined. The mRNA expression was measured by real-time quantitative PCR. All cytokines were determined in supernatant by ELISA kits for mice.Results:In the model control group, the levels of IL-4 and TNF-αwere increased significantly,but the levels of IL-2 and IFN-γwere decreased markedly. The gene expressions of IL-2 were also decreased and TNF-αwere increased. Xuebij ing treatment could significantly down-regulate the re-lease of IL-4,TNF-αand decrease in IL-2,IFN-γ.Conclusions:Treatment with Xuebijing injection might influence the polariza-tion of T cells in animals with SLE and induced Th cells to drift to Th1 cells.
Keywords：Systemic lupus erythematosusXuebij ing inj ectionT lymphocyteCytokine
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 28-32 )