Effects of intraperitoneal injection of suberoylanilide hydroxamic acid on organ function and visceral blood flow in rats with full-thickness scald injury involving 50% total body surface area
Zheng Jinguang
Hu Sen
Li Fangfei
Zhang Cong
Gao Mingjuan
Wang Xiaona
Yao Longqiang
Bai Xiaodong
Abstract：Objective:To investigate the protective effects of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA)against injury to organ function and visceral blood flow in rats with 50%total body surface area (TBSA)full-thick-ness scald injury.Methods:Forty-eight male SD rats,weighting 240-260 g,were randomly divided into three groups. In scald group,rats were subj ected to scald inj ury by immersing the back of the trunk for 1 5 seconds and the abdomen for 8 seconds in 100°C water,resulting in full-thickness injury involving 50% TBSA,then they received 0.25 ml of normal saline (NS) intraperitoneally. In SAHA group,rats were subj ected to same extent of inj ury,but they received an intraperitoneal inj ection of 0.25 ml of SAHA (7.5 mg/kg,dissolved in 4 ml of NS). In sham-scald group,rats were treated with 37 ℃ water,and then received 0.25ml of NS intraperitoneally. Blood flow of liver,kidney and small intestine was measured with Doppler blood flow meter. Abdominal aorta blood was drawn for measuring the levels of alanine aminotransferase (ALT),creatinine (Cr),creatine kinase MB (CK-MB),neuron specific enolase (NSE),and the activity of diamine oxidase (DAO)3 and 6 hours after inj ury. Results:The blood flow of liver,kidney,and intestine in SAHA group was significantly richer than that of the scald group 3 hours after burns(all P<0.05). The blood flow of the intestine in SAHA group was still higher signifi-cantly compared with that of scald group 6 hours after burn (all P<0.05).The contents of Cr,NSE and the activity of DAO in SAHA group were significantly lower than those of the sham-scald group 3 hours after burn (all P<0.05),and the con-tents of CK-MB,NSE and the activity of DAO in SAHA group were significantly lower than those of the scald group 6 hours after burns (all P<0.05).Conclusions:SAHA can improve the organ functions and intestinal blood flow after an exten-sive deep burn,and it protects the function of vital organs.
Keywords：Suberoylanilide hydroxamic acidHistone deacetylase inhibitorBurnsOrgan functionBlood flow
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 195-198 )