Study on induction of human bone marrow mesenchymal stem cells into human sweat gland-like cells

doi:10.3969/j.issn.1672-8521.2014.02.003

Abstract：Objective:To observe the changes in morphology and phenotype of human bone marrow mesenchymal stem cells (BM-MSCs)after co-cultured with human sweat gland cells (SGCs),in order to provide the experimental basis for the study on sweat gland cells induction-associated key transcription factors. Methods:BM-MSCs and SGCs were isolated,cultured, expanded and identified with the methods of differentiation to adipocytes and osteocytes invitro respectively. In transwell co-cultured experiment,BM-MSCs were induced into SGCs by indirect co-culture with 47 ℃heat-shocked confluent SGCs using transwell plate.But in transwell+inducing factors co-culture experiment,BM-MSCs culture medium was supplemented with series of inducing factors,including EDA-A1 ,recombinant human epidermal growth factor and ITS. After being co-cultured, morphological and phenotypic changes in BM-MSCs were observed. The antigen expressions of BM-MSCs were detected by immunofluorescence. Results:After co-cultured with heat-shock SGCs for 10 days,the shape of a part of MSCs changed from long-clostridial shape to flat and polygonal. Non-induced BM-MSCs were negative for CEA and CK1 9. Induced BM-MSCs were positive for CEA and CK19,and the positive cells of transwell + inducing factors co-culture was more abundant than that of transwell co-culture. Conclusions:MSCs could differentiate into sweat gland-like cells at phenotype and shape by a proper in vitro co-culture system of BM-MSCs and SGCs with or without inducing factors,and it was more efficient when they were co-cultured with inducing factors.