Study on pyruvate Ringer’s solution alleviates lipid peroxidative injury-induced multiple organ dysfunction during re-suscitation of hemorrhagic shock in rats

doi:10.3969/j.issn.1672-8521.2014.02.002

Abstract：Objective:To investigate the protective effects of pyruvate Ringer’s solution on lipid peroxidation in-jury-induced multiple organ dysfunction during resuscitation of hemorrhagic shock (HS)in rats. Methods:Hemor-rhagic shock was induced by withdrawing 45% of the calculated total blood volume within 20 minutes. Fifty-four SD rats were randomly divided into three groups (n=18). Group RN:HS with no fluid resuscitation. Group RL:HS resuscitated with commercial dl-lactate Ringer’s solution equal to 3 times the volume of shed blood. Group RP:HS resuscitated with pyruvate Ringer’s solution (pH 6.4)equal to 3 times the volume of shed blood. The mean arterial pressure (MAP)was monitored with PICCO cardiopulmonary capacity monitor,activities of Cu2+-Zn2+-superoxide dismutase (SOD)and the level of malondialdehyde (MDA)were determined 20 minutes before and 0,60,120,180,240,300,360 minutes after hemorrhage(T-20,T0,T60,T120,T180,T240,T300, T360).The content of MDA,oxidized and reduced glutathione,glutathione peroxidase,glutathione reductase and catalase in heart,lung,liver,kidney and j ej unum were determined at end of the experiment.Changes in creatine kinase (CK-MB),partial pressure of oxygen in artery (PaO2 ),alanine aminotransferase (ALT),diamine oxidase (DAO) and creatinine (Cr)were measured in arterial blood and plasma. The changes in water content in heart,lung,liv-er,kidney and jejunum were also measured.Results:At T120-360 after shock,the MAP in groups RL and RP was significantly higher than that of the group RN(P<0.05),and at T180-360,MAP in the group RP was signifi-cantly higher than that of the group RL(P<0.05). At T120-360,the blood Cu2+-Zn2+ SOD activities in group RP were significantly higher than that of group RN,but no significant difference was found when compared with those of the group RL. At T180-360,and the level of MDA was significantly lower in group RP than that in group RL(P<0.05). The content of MDA and oxidized glutathione in group RP was lower,but reduced glutathione was significantly higher than group RL in heart,lungs,liver and jejunum (P<0.05). The content of glutathione per-oxidase in group RP was lower but glutathione reductase was higher significantly than those in group RL in the heart,liver and jejunum (P<0.05). Protection of organ function and reduction of water content of tissues were pronounced in RP group as compared with RL group. Conclusions:Pyruvate Ringer’s solution may be significantly superior to lactate Ringer’s solution in alleviating multiple organ dysfunction and tissue edema induced by peroxida-tion after fluid resuscitation of HS.