Protective effects of suberoylanilide hydroxamic acid on scald-induced gut barrier dysfunction in rats
Tong Bin
Bai Xiaodong
Wang Jingjing
Zhao Zengkai
Luo Hongmin
Zhang Huiping
Lv Yi
Meng Xiangxi
Hu Sen
Abstract：Objective:To investigate the protective effect of histone deacetylase inhibitor,suberoylanilide hydroxamic acid (SAHA),on gut barrier dysfunction in rats with 50%TBSA full-thickness burns.Methods:Forty-eight male SD rats,weig-hing 240-260 g,were randomly divided into three groups:sham control group,scald group and SAHA group. Full thickness scald injury involving 50% TBSA was replicated by immersing the back and abdomen in 100 ℃ water for 15 and 8 seconds respectively in rats of scald group and SAHA group,and then 0.25 ml normal saline or 0.25 ml (7.5 mg/kg)SAHA was intraperitoneally injected,respectively. In the sham control group,37 ℃ water was used instead of 100 ℃ water,and 0.25 ml normal saline was intraperitoneally inj ected after sham inj ury. Blood flow (IMBF)of small intestine was measured by Doppler 3 and 6 hours after scald inj ury,while the activity of serum diamine oxidase (DAO)was determined,the expression of intestinal epithelial tight junction protein (ZO-1)of terminal ileum tissue was determined with immunofluorescence. Re-sults:In sham control group,IMBF was rich,the activity of DAO in serum was normal,the expression of intestinal epithelial ZO-1 was strong,presenting in a continuous state. Compared to sham control group,IMBF in scald group was significantly reduced by 58% at 3 hours and 75% at 6 hours after scald injury. The activity of DAO increased by 248% at 3 hours and 293% at 6 hours after scald injury. The expression of intestinal epithelial ZO-1 was weaker,and the fluorescence signal showed broken state. Compared to the scald group,IMBF in SAHA treatment group significantly increased by 44% at 3 hours and 45% at 6 hours after injury,and the activity of DAO was significantly decreased by 29%at 3 hours and 34%at 6 hours after inj ury,while the signal of intestinal epithelial ZO-1 was marked enhanced. Conclusions:SAHA has a significant protective effect on intestinal barrier function in rats suffering from severe burn inj ury.
Keywords：Suberoylanilide hydroxamic acidBurnsIntestinal barrierEpithelial tight junction protein
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 23-26 )