Comparison of biological characteristics of mesenchymal stem cells from normal and diabetic mice and their treatment effects on wound healing

Gao Dongyun; Gu Chengwei; Zhang Zhengzhong; Shen Tao; Fu Xiaobing; Wu Xu

doi:10.3969/j.issn.1672-8521.2014.01.003

Abstract：Objective:To explore the biological characteristics and effects on wound healing of mesenchymal stem cells (MSCs)from normal and diabetic mouse.Methods:The diabetic model was replicated by feeding with high fat (high sugar)chow for 1 month and receiving intraperitoneal injection of 40 mg/kg streptozocin for 3 days in mice. MSCs were isolated from bone marrow of normal or diabetic mice,and they were purified by Percoll gradient cen-trifugation and cultured invitro. The growth of normal and diabetic MSCs were observed under phase contrast mi-croscope,and their potential of differentiation to adipocytes and osteocytes were observed by oil red O or alizarin red staining. The proliferation of MSCs was investigated by enzyme-linked immunoadsorbent assay. The antigens on cell surface were detected by FACS and the ability of cell anti-apoptosis was assessed by using the detection kits. Sixteen diabetic mice were randomly divided into the normal MSCs treatment group and the diabetic MSCs treatment group(n=8).Circular full-thickness wounds with the diameter of which 8 mm were created on the dor-sum of mice. The mice in the two groups received subcutaneous injection of 2×106 either normal or diabetic MSCs around the wound. The wound healing process was assessed with sequential digital photographs.Results:The MSCs from diabetic mice group showed decreased expressions of CD2 9 and CD1 0 5 by FACS,with weaker capaci-ty of forming clones,proliferation and differentiation,and with weaker growth capacity,which appeared earlier compared to those of the normal MSCs. The normal MSCs exhibited better effects in promoting process of wound healing,especially during 7-14 days after injury. Conclusions:The diabetic MSCs have poorer biological character-istics and treatment effect on wounds compared with that of the normal MSCs. The results also suggest that dia-betic MSCs are not the worthwhile seed cells in treating diabetic wounds.