Effects of Water Management on Soil Enzyme Activitiesand Microbial Community Structure in Wheat Fields with Organic Fertilizer Application
ZHANG Chuangeng
GAO Yang
ZHANG Liming
SI Zhuanyun
LI Shuang
Abstract：[Objective]Study the effects of different water management measures on soil microbial community structure and soil enzyme activities in wheat field with organic manure.[Method]The changes of soil microbial community structure and soil enzyme activities under different fertilization treatment of full water supply and un-der water stress were analyzed in the field experiment.[Result]The activities of sucrase, alkaline phosphatase, cellulase, catalase and urease with sufficient water were significantly higher than those of the other treatment with water stress under the same fertilization, the activities of sucrase, alkaline phosphatase and cellulase could be significantly improved by the application of organic fertilizer, and the application of organic fertilizer had no significant effect on urease under the same water condition;the activity of catalase was enhanced by organic fer-tilizer under the condition of sufficient water supply. According to the Shannon and Simpson index, the communi-ty diversity of T1 and T2 was higher than that of T3 and T4. According to the Richness index, the richness of T1 was 31, and was increased by 15.01％, 40.90％and 72.22％than that of T2, T3 and T4, respectively. The princi-pal component analysis showed that the microbial community structure of different treatments was significantly different, and the water treatment had some effects on the rhizosphere microbial community structure actively. Ac-cording to the LEFSE analysis, phylum Firmicutes, order Myxococcales and order Sphingobacteriales had signifi-cant effect on T2 and T1;phylum Chloroflexi had significant effect on T3 and T4.[Conclusion]Application of organic fertilizer with full water supply could significantly improve the activities of sucrase, alkaline phospha-tase, cellulase and catalase and soil microbial diversity under the conditions of this experiment;and the sufficient irrigation is the premise to improve the soil enzyme activity and microbial diversity in wheat field, especially in the critical period of crop water and fertilizer requirement.
Keywords：organic fertilizerwater stresssoil enzyme activitiesmicrobial diversitymicrobial community structurePCALEFSE analysis
Publication Date：2018-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 38-44 )