Synthesis of hyaluronic acid-modifiel lanthanide coordination polymers and their targeted antitumor effects

QIU Wenlong; YE Xiaomei; CHEN Zhi; CHEN Mingxing; LIU Yulan; WU Dudu

doi:10.20227/j.cnki.2096-3610.2025.04.009

Abstract：Objective Preparation of a lanthanum coordination polymer with targeted antitumor effects.Methods A lanthanide coordination polymer antitumor drug delivery system(HA@DAL),modified by hyaluronic acid,was prepared by hydrothermal synthesis using lanthanum nitrate hexahydrate,hyaluronic acid(HA),and 4-dimethylaminobenzoic acid(DABA)as raw materials.Particle size distribution,zeta potential,thermogravimetric analysis(TGA),X-ray diffraction,infrared and ultraviolet spectroscopy were used to study the physicochemical properties of HA@DAL and the lanthanide coordination polymer(DAL).The anti-tumor effects of DAL and HA@DAL were compared using CCK8 assay,apoptosis analysis,and live cell imaging.The in vivo distribution of HA@DAL in tumor-bearing mice was examined by live animal imaging.Results DAL and HA@DAL were successfully prepared.The average particle size was(75.23±8.39)nm,the polydispersity index was 0.19±0.22,and the zeta potential was(-30.60±3.48)mV.The fluorescence characteristics of lanthanum metal were retained.Cytotoxicity and apoptosis assays showed that HA@DAL possessed stronger antitumor activity compared with DAL(P<0.01),and exhibited similar cytotoxicity compared with the same dose of DOX(P>0.05).In vitro live cell imaging results showed that DAL could be taken up by cells,causing them to emit blue fluorescence but lacked selectivity,whereas HA@DAL had good tumor-targeting ability.Animal live imaging results showed that HA@DAL could reach the tumor site precisely.Conclusion The prepared HA@DAL has promising prospects for tumor-targeted therapeutic applications and provides an experimental basis for the development of novel nanodrug delivery systems.