Isolation of butyric acid lactone compounds from marine-derived Aspergillus terreus and evaluation of their anti-neuroinflammatory activity
YANG Qing
JIANG Zhongping
Abstract：Objective To investigate into the butenolides and their anti-neuroinflammatory activities derived from the marine-associated fungus Aspergillus terreus.Methods Normal-phase and reversed-phase silica gel column chromatography and semi-preparative high performance liquid chromatography were employed to isolate the compounds;Structural elucidation was determined using nuclear magnetic resonance spectroscopy(NMR),mass spectrometry(MS),and calculated and experimental electronic circular dichroism(ECD)data;The isolated compounds were evaluated for their inhibitory effects on nitric oxide(NO)production in lipopolysaccharide(LPS)-activated BV-2 cells.A blank control group,a model group(with LPS added),an administration group(with both LPS and the compound added),and a positive control group(with LPS and dexamethasone added)were set up for the test.Results Six butenolide derivatives(1-6)were isolated and identified,including one novel compound(1).Compared with the blank control group,the NO content in LPS-activated BV-2 cells of the model group was significantly increased(P<0.001);In comparison to the model group,the NO content in the cell culture medium of the compounds 2,3,4,6 were significantly decreased(P<0.001 or P<0.005).However.no significant difference was observed in the NO content in the compound 5 group.Conclusions A novel butenolide derivative(compound 1)was isolated from the marine-derived fungus Aspergillus terreus,while compounds 2-4 and 6 exhibited weak anti-neuroinflammatory activities.
Keywords：marine-derived fungusbutenolidesisolationstructural identificationanti-neuroinflammatory activityAspergillus terreus
Publication Date：2025-08-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 355-361 )