An association between gut microbiota and clinical parameters in very/extremely low birth weight infants during the first 28 days of life
LIU Ling
LI Ye-shan
CHEN Rou-yi
AO Dang
LIN Shao-zhu
YANG Yu-ping
Abstract：Objective To investigate the association between gut microbiota and clinical indicators in very/extremely low birth weight(V/ELBW)infants within the first 28 days of life.Methods Preterm infants with<32 weeks'gestational age and<1.5 kg birth weight were recruited.Their stool samples were collected on days 1,14 and 28,and then subjected to 16S rRNA sequencing and LC-MS/MS analysis.The relationship between gut microbiota and metabolites and clinical factors was investigated by Spearman correlation analysis.Results The time to full enteral feeding had the greatest influence on the gut microbiota(r2=0.105,P=0.001).The time to full enteral feeding was positively correlated with Staphylococcus,Dubosiella,Lactobacillus,Faecalibacterium,Acinetobacter johnsonii,Faecalibaculum rodentium,and negatively with Clostridium paraputrificum and Clostridium butyricum(P<0.05).Gut microbiota was positively associated with gestational age and cesarean section on D1,and negatively with positive pressure ventilation time,antibiotic course,and time to full enteral feeding on D14 and D28(P<0.01).12-hydroxyoctadecanoic acid,succinic acid,5-aminopentanoic acid and dodecylbenzene sulfonic acid were negatively correlated with the time to full enteral feeding(P<0.05).Conclusion There is a significant correlation between gut microbiota and metabolites,and clinical indicators.The time to full enteral feeding has the greatest impact on early-stage gut microbiota of V/ELBW infants.
Keywords：gut microbiotavery/extremely low birth weight infants16S rRNA sequencingfull enteral feedingLC-MS/MS
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 406-412 )