Protective effect of Shenqi Fuzheng liquid on high glucose-induced myocardial injury using network pharmacology and in vitro experiment
LI Qin
HE Xu-wei
ZHANG Yong-gang
WEI Wen-bin
CHEN Ji-ming
Abstract：Objective To explore the potential mechanism of Shenqi Fuzheng liquid(SFL)on high glucose-induced myocardial injury(MI).Methods The active ingredients and targets of SFL,and MI genes were screened from TCMSP,GeneCards and OMIM databases,respectively.A PPI network was constructed using String database,and the intersection genes were analyzed by GO and KEGG using Metascape database.H9c2 myocardial cells were treated with 35 mmol/L glucose or adding with 1 and 5 g/L SFL.Survival rate,apoptosis and PI3K/AKT expression were detected by MTT,fluorescence staining and Western blot,respectively.Results There were 495 active ingredients of SFL,2 148 genes of MI,and 227 intersecting genes between them.These intersecting genes were mainly involved in endocrine resistance,HIF-1,AMPK,PI3K/AKT signaling pathways.SFL increased survival rate,reduced apoptosis,and upregulated p-PI3K/p-AKT expression of injured myocardial cells.Conclusion SFL can alleviate high glucose-induced MI through PI3K/AKT signaling.
Keywords：Shenqi Fuzheng liquidmyocardial injurynetwork pharmacologyPI3K/AKT signaling
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 384-389 )