Study on the anti-rotavirus effect of different extracts of Valeriana jatamansi Jones
LI Feng
SONG Li-jun
ZHOU Yu-jing
FENG Yu-xuan
YUAN Yue
QIAN Yu-pei
YANG Si-yan
YI Hao-sen
YANG Zhi-yan
YU Run-yu
ZHAO Wen-chang
Abstract：Objective The aim of this study is to examine the anti-rotavirus(RV)effects of different extracts of Valeriana jatamansi Jones.Methods The RV-Wa strain infected MA104 cell model was used,and the virus inhibition rate was determined by using MTT staining.The in vitro anti-RV activity of spider perfume extract,70%ethanol extract(ZZX-1)and 90%ethanol extract(ZZX-2)was purified by the macroporous adsorption resin method.Immunofluorescence and real-time fluorescence quantitative PCR(qRT-PCR)were used to detect the expression of RV structural protein VP6,and to verify the anti-RV effect of spide.Caco-2 cells were infected with RV-Wa strain and the effects of arachnid on the expressions of neurotrophic factor GDNF,tight junction protein ZO-1 and pro-inflammatory factors(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)were detected by qRT-PCR.Results The extract of Valeriana jatamansi Jones did not have any anti-RV effect;Valeriana jatamansi Jones extracts ZZX-1 and ZZX-2 have anti-RV biosynthesis effects.The results of immunofluorescence and qRT-PCR indicate that both ZZX-1 and ZZX-2 can reduce the fluorescence and mRNA expression of VP6,and inhibit RV synthesis.Compared with the RV group,ZZX-1 and ZZX-2 significantly increased the expression levels of neurotrophic factor GDNF and tight junction protein ZO-1 mRNA(P<0.001 or 0.01),and reduced the relative expression levels of tumor necrosis factor alpha(TNF alpha),interleukin-1β(IL-1β),and interleukin-6(IL-6)mRNA(P<0.0001 or 0.001).Conclusion The alcohol extracts ZZX-1 and ZZX-2 from the traditional Chinese medicine Valeriana jatamansi Jones have anti-RV biosynthesis effects,which may exert anti-RV infection effects by increasing the expression of GDNF and ZO-1 and reducing the expression of inflammatory factors IL-1 β,IL-6,and TNF-α.
Keywords：rotavirusValeriana jatamansi Jonescyclenyl ether terpenesintestinal epithelial cellstight junction protein
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 356-364 )