Breeding of Scamp2-/-/ApoE-/-mice and their detection of serum high density lipoprotein cholesterol and hepatic ABCA1 levels
HONG Wei-tao
CHEN Geng-ji
ZHANG Peng-fei
CHEN Xiao-jia
DING Hang
MA Wei-lie
ZHANG Zhi-zhen
Abstract：Objective To breed Scamp2-/-/ApoE-/-mice and detect their serum high density lipoprotein cholesterol(HDL-C)and hepatic ABCA levels.Methods Scamp2-/-and ApoE-/-mice were raised in SPF room,and Scamp2-/-/ApoE-/-mice were bred using F0 homozygous hybridization and F1 heterozygous and F2 homozygous self-inbreeding.Genotypes of offspring were identified by PCR amplification and agarose gel electrophoresis.Serum HDL-C and hepatic ABCA1 mRNA levels were determined.Results Scamp2-/-ApoE-/-,Scamp2-/-/ApoE+/+and Scamp2+/+/ApoE-/-mice were successfully bred.Serum HDL-C and hepatic ABCA1 mRNA levels were lower in Scamp2-/-,Scamp2-/-and Scamp2-/-/ApoE-/-mice than in WT mice(P＜0.001).Compared with ApoE-/-mice,serum HDL-C and hepatic ABCA1 mRNA levels were respectively decreased by 25.26％and 21.48％in Scamp2-/-/ApoE-/-mice(P＜0.01).Conclusion Scamp2-/-/ApoE-/-mice are successfully obtained.Scamp2 knockout can reduce hepatic ABCA1 and serum HDL-C contents in mice.
Keywords：Scamp2ApoEgene knockouthigh density lipoprotein cholesterolABCA1
Publication Date：2024-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 141-145 )