Mechanism of anti-gastric cold effect of galangal-dried ginger extract on gastric cold model mice

LI Ze-sen; LI Cheng-long; WEN Zhen-rong; OU Jian-xia; LAI Jun; HUANG Li-ping

doi:10.3969/j.issn.1005-4057.2023.06.002

Abstract：Objective To compare therapeutic effects of galangal-dried ginger extract of different proportions on stomach cold and provide evidence for the pharmacodynamic study on galangal-dried ginger extract in regulating gastrointestinal function.Methods The gastric cold syndrome model mice were established by intragastric administration of ice water and NaOH.The mice were randomly divided into 9 groups(n=10):Normal Group,Model Group,Motilium Group,Fuzi Lizhong Pill Group,Dried Ginger Group,Galangal Group and High-,Medium-and Low-Dose Galangal+Dried Ginger Groups(hereinafter referred to as High-,Medium-and Low-Dose Combination Groups).All the mice received continuous administration for 30 d,once a day.The changes of tongue manifestation,abdominal temperature and body weight of mice were observed and recorded.The gastric residual rate,intestinal propulsion rate and organ indexes of mice were calculated.The levels of gastrin(GAS),motilin(MTL),acetylcholine(ACh),superoxide dismutase(SOD),malondialdehyde(MDA)and interleukin 6(IL-6)in gastric tissue were measured by ELISA.The pathological morphology of gastric tissues in each group was observed.Results Compared with the Normal Group,the gastric histology of the Model Group presented gastritis symptoms,and the levels of GAS,MTL,ACh and SOD in the gastric tissue of the Model Group were significantly decreased,the levels of MDA and IL-6 of the Model Group were significantly increased(P＜0.01).After administration,compared with the Model Group,the gastric histopathology of High-,Medium-and Low-Dose Combination Groups showed alleviated gastritis symptoms,the tongue manifestation of High-,Medium-and Low-Dose Combination Groups showed faint red and glossy,the levels of GAS,MTL,ACh and SOD,the intestinal propulsion rate and gastric evacuation rate,body temperature and body mass level of High,Medium-and Low-Dose Combination Groups were increased,and IL-6 levels of High-,Medium-and Low-Dose Combination Groups decreased significantly(P＜0.05 or 0.01).Conclusion Combined medication of galangal and dried ginger can significantly improve the gastric cold symptom,increase the body temperature,and promote the gastrointestinal motility of mice,which may be closely related to the up-regulation of GAS,MTL,ACh and SOD levels and the down-regulation of MDA and IL-6 levels.