Effect of MACC5 isolated from traditional Chinese medicine on the rats with MsPGN induced by anti-Thy1.1

doi:10.3969/j.issn.1005-4057.2001.03.003

Abstract：Objective:To observe the effect of MACC5 isolated from traditional Chinese medicine on the rat with mesangial proliferative glomerulonephritis(MsPGN)induced by anti-Thy1.1 antibody.Methods:Anti-Thy1.1 serum(ATS)was produced,and then injected intravenously to SD rats in order to induce experimental model with MsPGN.The rats with MsPGN were randomly divided into 3 groups,and each group contained 5 rats.Both of experimental groups were injected peritoneally with MACC5 1.0 mL and 3.0 mL,respectively,whereas control group received a peritoneal injection of normal saline 1.0 mL .Urine volume and urinary protein were examined everyday. The rat was killed on the 11th day,and the expression of PCNA in the glomeruli was detected by immunohistochemistry method.Results:The urine volume of experimental groups was more than that of control group (P<0.05),the urinary protein and the amount of GMC of experimental groups were significantly lower than those in control group (P<0.01-0.001),and there existed a difference in the expression of PCNA between MACC5 3.0 mL group and control group (P<0.05).Conclusion:It is suggested that MACC5 possesses effects of diuresis and reduction of urinary protein excretion,and promotes the repairing process of mesangial lesions of MsPGN.