Effects of cryoprotectants on viability of cryopreservative corneal endothelial cells
WU Bin-bin
HUANG Ting
Abstract：Objective：To investigate effects of osmotic stress of cryoprotectants on viability of cryopreservative corneal endothelial cells．Methods：New-born bovine corneas were divided into two groups randomly，and then cryopreserved with the simplified “two-step” method．Human serum albumin（HSA） and corneal-potassium-TES（CPTES） were chosen as cryoprotectant vehicles in group Ⅰ and group Ⅱ，respectively．The indexes，including cellular density，survival rate，polymegathism and pleomorphism，were compared respectively after thawing between two groups．Results：There were no significant differences on cellular density and survival rate between two groups，while group Ⅱ was superior to group Ⅰ on both polymegathism and pleomorphism．Conclusion：CPTES solution，an important ingredient of cryoprotectant，is helpful in improving the viability of cryopreservative corneal endothelial cells．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 92-93 )