Study on the rat with mesangial proliferative glomerulonephritis induced by anti-Thy 1．1
Abstract：Objective：To explore the way to reproduce a rat model with mesangial proliferative glomerulonephritis（MsPGN） induced by anti-Thy 1．1，and to investigate its clinicopathologic characteristice and the expression of PCNA and Bcl-2 in the mesangial area．Methods：Anti-Thy1．1 serum（ATS） was produced in New Zealand white rabbits by immunization with SD rat thymocytes，and then injected intravenously to induce SD rat model with MsPGN．Proteinuria was measured everyday，the degree of mesangial proliferation was observed by HE，PASM and Masson’s stains，and the expression of PCNA and Bcl-2 in the mesangial area was detected by LSAB method．Results：The titre of ATS could amount to 1∶3 200，and the optimal dosage to induce rat model was 0．01 mL／g*wt．The rat model was characterized by heavy proteinuria，mesangial proliferation，and enhanced expression of PCNA and Bcl-2 in the mesangial area．Conclusion：A stable and typical rat model with MsPGN can be successfully induced by ATS．The immune response and inhibition of apoptosis of mesangial cells may play an important role in the mesangial proliferation of the rat model with MsPGN．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 87-89 )